FINAL DAY!

Lab Day 12

Today we figured out what our unknown bacteria is. After taking all of our test results and using flow charts we discovered that our bacteria is E. coli.

Our flow chart consisted of the flowing steps:

Gram-negative rod-shaped ID flowchart

Oxidase Test—Negative

Lactose Test—Positive

Indole Test—Positive

Citrate Test—Negative

    E. coli.

Joanna Dawyot, Cassie Livingston, Mary Rose Capara 

 

DisclaimerAll content provided on this blog is representation of the blog owner and not FranciscanUniversity of Steubenville. The information on this site is purely used for education purpose. The owner of this blog makes no representations as to the accuracy or completeness of any information on this site or found by following any link on this site. The owner will not be liable for any errors or omissions in this information nor for the availability of this information. The owner will not be liable for any losses, injuries, or damages from the display or use of this information. Privacy The owner of this blog does not share personal information with third-parties nor does the owner store information is collected about your visit for use other than to analyze content performance through the use of cookies, which you can turn off at anytime by modifying your Internet

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Lab Day 11

Lab Day 11

We looked at the results from the Steph. Blood Agar Plate incubated with the Bacitracin, which showed a positive for Strep. There was beta-hemolysis and it was sensitive to the Bacitracin.

Next we looked at our unknown sample tested with the neosporin:

The neomycin was effective on our bacteria, which agrees with the data taken from the penicillin test.

Next we completed an ELISA test on given samples to see how we test for antigens

  This test is used to detect antibodies in the blood. This test is often used to see if you have been exposed to viruses or other substances that cause infection. It is often used to screen for current or past infections. 

The bottom of each well is coated with a protein to which will bind the antibody you want to measure. Whole blood is allowed to clot and the cells are centrifuged out to obtain the clear serum with antibodies (called primary antibodies). The serum is incubated in a well, and each well contains a different serum (see figure below). A positive control serum and a negative control serum would be included among the 96 samples being tested.

After some time, the serum is removed and weakly adherent antibodies are washed off with a series of buffer rinses. To detect the bound antibodies, a secondary antibody is added to each well. The secondary antibody would bind to all human antibodies and is typically produced in a rodent. Attached to the secondary antibody is an enzyme such as peroxidase or alkaline phosphatase. These enzymes can metabolize colorless substrates (sometimes called chromagens) into colored products. After an incubation period, the secondary antibody solution is removed and loosely adherent ones are washed off as before. The final step is the addition the enzyme substrate and the production of colored product in wells with secondary antibodies bound.

Then we looked at our results for both of the Agar Disk Diffusion Assay.

 The reaction reacts and makes precipitate- anti bovine album.

Finally we placed samples from all of our bacteria into a beaker of distilled water. Then to test a way of killing bacteria we used UV light to purify the water.

Joanna Dawyot, Cassie Livingston, Mary Rose Capara 

 

DisclaimerAll content provided on this blog is representation of the blog owner and not FranciscanUniversity of Steubenville. The information on this site is purely used for education purpose. The owner of this blog makes no representations as to the accuracy or completeness of any information on this site or found by following any link on this site. The owner will not be liable for any errors or omissions in this information nor for the availability of this information. The owner will not be liable for any losses, injuries, or damages from the display or use of this information. Privacy The owner of this blog does not share personal information with third-parties nor does the owner store information is collected about your visit for use other than to analyze content performance through the use of cookies, which you can turn off at anytime by modifying your Internet

 browser’s settings. The owner is not responsible for the republishing of the content found on this

 blog on other Web sites or media without permission.Blog CommentsThe owner of this blog reserves the right to edit or delete any comments submitted to this blog without notice due to;1. Comments deemed to be spam or questionable spam2. Comments including profanity3. Comments containing language or concepts that could be deemed offensive4. Comments that attack a person individuallyThis policy is subject to change at anytime.