Lab Day 6

Part 1-Observations

Today in our lab we observed the results from the food tests that we did the day before. Our results are going to help us figure out what our unknown sample of bacteria is.

We also looked at our virus test that we did yesterday. Our "Europe" did not turn out very well. The other group's "Awkward", however, turned out very well. 

The first test we checked is the starch plate. A positive test would show a halo on the plate around the bacteria. This halo did not show up on our plate, therefore our bacteria had a negative starch test. We used Gram's Iodine to check to see the halo. 

The next test was the skim milk test, which tested caesin. This was a negative result for us. Dr. P told us to incubate the plate longer. We shall see more results tomorrow.

The lipid, fat, plate was the next plate we observed. There is clear zones due to break down of the lipids. This test was negative for us.

A positive DNA plate when loaded with N. HCL will show a dark halo. Our plate did not show a dark halo when the solution was added, therefore our DNAase test was negative.

The next set of tests we observed were found in the tubes.

Sucrose Test: negative

Litmus Milk: lactic acid curds milk, pink hue, cracks in the curd meaning that there was gas produced. We had a positive test result, however, Dr. P told us to re-incubate.

Maltose: positive

Glucose: positive. There was color change

Lactose: positive. Change in the color

Triple Sugar IA: positive. Both the slant and the butt showed a yellow color which meant that glucose, lactose, and/or sucrose were fermented. Meaning that the slant and the butt were acidic.

Part 2-Tests

The next part of the lab that we did was complete more tests on our bacteria.

Oxidase Test: to determine if our bacteria has cytochrome oxidase a participate in electron transport chain during respiration.

We took the lid of our bacteria plate placed filter paper on it. Then we squeezed two drops of oxidase reagent and then we added the bacteria. The bacteria turned yellow not purple on contact with the reagent, therefore, the results were negative.

The next set of test that we did are going to set overnight to incubate.

We inoculated 5 different tests.

Urea

Citrate

Nitrate

MR-UP

Protein Indole

Today during lab we noticed that Dr. P had a cut on his hand. He showed us a cool way to decrease the bleeding of his cut. He took a piece of chalk and crushed it and the placed the chalk on the cut. This because there is calcium in the chalk the coagulation increased.

Joanna Dawyot, Cassie Livingston, Mary Rose Capara 

DisclaimerAll content provided on this blog is representation of the blog owner and not FranciscanUniversity of Steubenville. The information on this site is purely used for education purpose. The owner of this blog makes no representations as to the accuracy or completeness of any information on this site or found by following any link on this site. The owner will not be liable for any errors or omissions in this information nor for the availability of this information. The owner will not be liable for any losses, injuries, or damages from the display or use of this information. Privacy The owner of this blog does not share personal information with third-parties nor does the owner store information is collected about your visit for use other than to analyze content performance through the use of cookies, which you can turn off at anytime by modifying your Internet

 browser’s settings. The owner is not responsible for the republishing of the content found on this

 blog on other Web sites or media without permission.Blog CommentsThe owner of this blog reserves the right to edit or delete any comments submitted to this blog without notice due to;1. Comments deemed to be spam or questionable spam2. Comments including profanity3. Comments containing language or concepts that could be deemed offensive4. Comments that attack a person individuallyThis policy is subject to change at anytime.