Our Trip to the
Gym Turns Scientific and Inoculation of Bacteria
May 14, 2014
Have you ever
noticed the signs at the gym saying “Wipe down your machines once
you are done with them”? When studying Medical Microbiology, Dr.
Pathakumuri asked us to find a sample of bacteria on the campus. We
took this opportunity to help people understand why wiping down
machinery is so important.
We left the
lab in our white lab coats ready to obtain our specimen. Upon
entering the gym, we went to the most commonly used piece of
equipment, the squat bar. To collect our sample, we practiced aseptic
technique, using an agar broth and a cotton swab. Then we transferred
the bacteria to an agar plate using the streak method, rotating the
plate a quarter around every time we streaked the bacteria. Once we
arrived back to the lab we placed the plate in an incubator that was
set at 24 degrees C.
Inoculation of
Bacteria.
Hypothesis:
We believe that our unknown sample of bacteria that Dr. P gave to us
affects the body in some way, being that the human body temperature
is 37 degrees C as is the sample bacteria that we were given. Whether
it has a beneficial purpose or detrimental affect, we are unsure of
at the time being.
Our
Goal: To make a pure stock.
Materials
Used:
Loop
2
Agar Slant Tubes
Agar
Plate
Bunsen
Burner
Agar
Broth
Gloves
Documentation
Device
A.
Transferring bacteria from a slant agar to a plate
Taking
the loop we passed it through the tip of the inner cone of the flame
to ensure sterilization. After we passed the mouth of the tube
through the flame we dipped the loop into the bacteria sample. Then
we used quadrant technique and the streak method, we spread the
bacteria on the plate. After we finished each quadrant we sterilized
the loop and then streaked the bacteria from the previous quadrant
into the next one. This method ensures that we will grow a pure
culture.
B. Transferring bacteria from a slant agar to a slant agar
Practicing
aseptic technique throughout, we used the loop to transfer bacteria
from one slant to the other. We did a shallow stab to collect the
bacteria from the contaminated sample. Using a snake-like motion, we
spread the bacteria onto the pure slant. We repeated this technique
twice.
- Transferring bacteria from a slant agar to agar broth Practicing aseptic technique, we used the loop to transfer bacteria from the slant to the broth. We did another shallow stab to collect a small sample colony from the contaminated sample. We dipped the loop with the bacteria in to the broth.
We then placed all 4 of the samples into an incubator at 37 degrees C.
Joanna Dawyot, Cassie Livingston, Mary Rose Capara
DisclaimerAll
content provided on this blog is representation of the blog
owner and not FranciscanUniversity of Steubenville. The
information on this site is purely used for education purpose.
The owner of this blog makes no representations as to the
accuracy or completeness of any information on this site or found by
following any link on this site. The owner will not be liable
for any errors or omissions in this information nor for the
availability of this information. The owner will not be liable for
any losses, injuries, or damages from the display or use of this
information. Privacy The owner of this blog does not share
personal information with third-parties nor does the owner store
information is collected about your visit for use other than to
analyze content performance through the use of cookies, which you
can turn off at anytime by modifying your Internet
browser’s
settings. The owner is not responsible for the republishing of the
content found on this
blog
on other Web sites or media without permission.Blog CommentsThe owner
of this blog reserves the right to edit or delete any comments
submitted to this blog without notice due to;1. Comments deemed
to be spam or questionable spam2. Comments including profanity3.
Comments containing language or concepts that could be deemed
offensive4. Comments that attack a person individuallyThis policy is
subject to change at anytime.
No comments:
Post a Comment