Sick Days and Bacteria Tests

Day 4
May 19, 2014

Today was the day we all found out about our acceptance into the nursing program!! Lab began with Dr. P teasing Elizabeth about her acceptance. She was afraid she didn't get in and didn't want to get her letter from Dr. Miller but in the end, we got her to smile with the good news.

Saturday night, Pete, Dr. P, Mary Rose, Ben, and Adrianna went to the lab to take a culture of Mary Rose's throat, thinking that she had strep. The plate with the swab had incubated over two nights and we were ready to see the results.

The plate had obvious bacterial growth and as it turns out, Mary Rose tested positive for strep. Pete had experimented with the strep by adding Penicillin, cinnamon oil, and clove oil to the plate. We were able to link lecture to lab by observing the changes in the bacteria. The cinnamon oil killed RBC but did the best job of killing the strep. It did a better job at this than Penicillin! Clove oil also did an incredible job at killing the bacteria as did Penicillin. We were able to deduce the bacteria's death due to clear rings around each of the three substances on the plate.

Now we know! If you have strep or a throat infection, take cinnamon and clove!!

Part 1: Thioglycolate

Practicing Ascetic technique throughout the experiment, we took a loop and, taking a culture of our unknown bacteria, we dipped it into a test tube filled with thioglycolate. This substance was pink towards the top of the tube and a yellowish color on the bottom. This was due to the thioglycolate being oxygenated on the top and not the bottom. By stabbing our bacteria into this tube, we will be able to see if our bacteria is aerobic or anaerobic due to how it will grow in the incubator overnight.

Upon receiving Dr. P's permission, we repeated this procedure with Mary Rose's strep culture. This too we put into the incubator.

Part 2: Stab to test Motility

This we did by taking our loop with our unknown bacteria and stabbing it into an agar deep provided by Dr. Pathakamuri. We put it into the incubator and we'll see what happens tomorrow!

Part 3: Inoculation of a Slant Agar

Because our first slant samples melted we had to redo the two agar slant samples we did earlier last week. Taking our bacteria with a loop, and practicing ascetic technique throughout, we used the snake method to spread the bacteria on the surface of an agar slant. We'll see what happens to the bacteria tomorrow!

Part 4: Testing the effects of a virus on bacteria

We began this procedure by spreading 50ml of group 1's, our group, broth onto a plate. This we did by using a dispenser set to gather 50ml of the broth. Dr. Pathakamuri gave each of the students a small amount of an unknown virus and, taking a big tip, we each wrote something on the broth with it. Our group chose to write Europe! We're excited to be going officially now that we're                      in the program!

After that, we put the plates the incubator for viral growth.

Part 5: Culturing Anaerobic Bacteria

Taking a small sample of our bacteria, we spread this on a plate. We then put the plate into an anaerobic jar. This jar is used to test whether or not our bacteria is anaerobic. This jar expels all traces of oxygen by the use of hot gas packs in the air tight container. We also put an indicator strip into the box. This will indicate the presence or lack of oxygen. Pale white = no oxygen and blue = presence of oxygen.

We'll get all of our results tomorrow!!

  

Joanna Dawyot, Cassie Livingston, Mary Rose Capara 

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