Lab Day 9

Lab Day 9

Today we took a series of samples from members of our group.

Nasal Swab: For this test we took a swab sample, using aseptic technique, and smeared the sample on a Mannitol-Salt plate because we are testing for MRSA/staph. When taking the sample we needed to use a saline solution, because the area being tested is not moist, and then rotated the swab 2-5 times counterclockwise and clockwise.

Throat Swab: For this test we also swabbed the back of the throat using aseptic technique. In this test we are testing for strep, therefore we swabbed the sample on a Blood Agar plate.

Urine Swab: For this test, a member in our group preformed a urine test. We then placed some of the urine on a EMB plate, because in this type of test we are testing for E. coli we use either an EMB or MacConkey plate, and used a sterilized loop.

After we preformed these tests we placed these plates in the incubator and we shall observe our results tomorrow.

Next we started our Antibiotic Sensitivity Test. We spread our unknown bacteria onto a new nurtient agar plate. Then we added several antibiotics onto the plate. We sterilized tweezers using 95% ethanol.

Antibiotics

1.  Neomycin
2. Tetracytine
3. Penicillin
4. Lemon Oil
5. Cinnamon Oil
6. Agumented

Tomorrow we shall see how our bacteria reacts to specific antibiotics.

Joanna Dawyot, Cassie Livingston, Mary Rose Capara 

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